Transmission interference microscopy of anterior human eye
Résumé
We demonstrate that the fundamental ideas of E. Abbe about the interference nature of transmission microscopy are relevant for imaging of in vivo human eye. The transmission geometry in the eye is achieved by projecting illumination onto the posterior eye (sclera) and using the back-reflected light as a secondary illumination source for anterior eye structures. In particular, we show that the tightly localized illumination spot at the sclera acts as a closed condenser aperture in conventional microscopy, significantly enhancing interference contrast. In vivo cells and nerves are revealed with increased contrast across all corneal layers and within an extended 2 mm field of view, 7× larger than the clinical state-of-the-art. This transmission microscope presents the only modality, where the crystalline lens epithelial cells, fibers and sutures can be readily counted. In patients, Fuch's endothelial dystrophy, a major ophthalmic disease affecting 300 million people, is highlighted under a new transmission contrast. Finally, the instrument is built using consumer cameras, which opens opportunities for its wide adoption in the pre-screening and follow-up of cataract/refractive surgeries and the diagnosis of corneal infections in developing countries, where anterior eye diseases are most prevalent.
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OTT paper15.pdf (2)
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video1_simulation-propagation.mov (13)
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video2_ContrastDependency-Illumination_ScaleBar50um.mov (29)
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video3_FlyThrough-ScaleBar50um.mov (28)
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video4_Nerves_ScaleBar-100um.mov (21)
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video5_2phaseTomography_ScaleBar100um.mov (5)
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